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Characterization and genus identification of rhizobial symbionts from Caragana arborescens in western Canada [r-libre/479]

Moukoumi, Judicaël; Hynes, Russell K.; Dumonceaux, Timothy J.; Town, Jennifer, & Bélanger, Nicolas (2013). Characterization and genus identification of rhizobial symbionts from Caragana arborescens in western Canada. Canadian Journal of Microbiology, 59 (6), 399-406. https://doi.org/10.1139/cjm-2013-0158

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Content : Accepted Version
Item Type: Journal Articles
Refereed: Yes
Status: Published
Abstract: Naturally occurring nitrogen-fixing symbionts from root nodules of caragana (Caragana arborescens) growing in central Saskatchewan were isolated following surface sterilization of caragana root nodules and squashing and spreading of the contents on yeast extract – mannitol medium. The symbiotic nature of the strains was confirmed following inoculation onto surface-sterilized C. arborescens seed in a gnotobiotic Leonard jar system. The Rhizobium isolates from C. arborescens root nodules were intermediate in generation time (g) (mean g of 5 isolates was 6.41 h) compared with the fast growers, Rhizobium leguminosarum NRG457 (g: 4.44 h), Rhizobium tropici 899 (g: 3.19 h), and Sinorhizobium meliloti BALSAC (g: 3.45 h), but they were faster than the slow-growing Bradyrhizobium japonicum USDA 110 (g: 13.86 h) and similar to Mesorhizobium amorphae (g: 7.76 h). Nitrogen derived from fixation by measuring changes in delta15N natural abundance in plant tissue confirmed the effectiveness of the strains; approximately 80% N2 from fixation. Strain identification was carried out by determining the sequences of 3 genes: 16S rRNA-encoding genes, cpn60, and recA. This analysis determined that the symbiotic partner of Canadian C. arborescens belongs to the genus Mesorhizobium and seems more related to M. loti than to previously described caragana symbionts like M. caraganae. This is the first report of Mesorhizobium sp. nodulating C. arborescens in western Canada.
Official URL: http://www.nrcresearchpress.com/doi/abs/10.1139/cj...
Depositor: Bélanger, Nicolas
Owner / Manager: Nicolas Bélanger
Deposited: 11 Nov 2014 18:16
Last Modified: 16 Jul 2015 00:46

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